A SSR Kit to study Sorghum genetic diversity
A SSR Kit to study Sorghum genetic diversity
The sorghum SSR kit has been elaborated in the frame of a large genotyping project (3000 sorghum accessions x 48 SSR loci) supported by the Generation Challenge Program , Sub-Programs 1 and 5 (grants dedicated to Daniel Fonceka and M'Baye N'Doye Sall, Ceraas, Senegal). It is composed of the allelic sizes of 3 controls for each of the 48 SSR locus.
Composition of
the controls:
includes the accession number (as provided by ICRISAT), in the genotyping
order, and there belonging to the controls. Characteristics of the 48 markers:
includes, for each locus, its name,
the microsatellite motive, forward and reverse primer sequences, the number of alleles
observed among the controls and their size range, and linkage group assignation (following Kim
et al. (2005) nomenclature).
Allelic patterns of control samples: shows the Licor IR2 locus image,
allelic content of each sample and of the controls.
All experiments related to this kit were performed on the
Languedoc
Roussillon Genotyping Platform, hosted by the
CIRAD, under the supervision of Claire Billot and
with the collaboration of Ronan Rivallan, Jean-Francois Rami and Monique Deu. |
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